Journal: Clinical and Experimental Immunology

Article Title: The effects of maternal anti-alpha-enolase antibody expression on the brain development in offspring

doi: 10.1093/cei/uxac086

Figure Lengend Snippet: the levels of ENO1Ab in the H-ENO1Ab dams and their pups. Serum and amniotic fluid samples were collected as depicted in . Anti-ENO1 total IgG and its subtypes were measured by enzyme-linked immunosorbent assay (ELISA). The results are shown above for the dams (A, n = 6-14/group, ** P < 0.01, *** P < 0.001 vs. the control group) and the pups (B, n = 14-19 for total IgG, n = 5-8 for IgG1, n = 5-9 for IgG2a, IgG2b and IgG3, ** P < 0.01, *** P < 0.001, not significantly (NS) vs. the pups of the control group).

Article Snippet: After 2 h incubation at RT followed by repeated washing, the plates were further incubated with diluted horseradish peroxidase (HRP)-labeled goat anti-mouse total IgG (1:200 diluted for TgAb;1:10 000 for ENO1Ab), IgG1(1:1000 for ENO1Ab), IgG2a (1:1000 for ENO1Ab), IgG2b (1:1000 for ENO1Ab), or IgG3 antibodies (1:1000 for ENO1Ab), which were all from Bethyl (USA).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Clinical and Experimental Immunology

Article Title: The effects of maternal anti-alpha-enolase antibody expression on the brain development in offspring

doi: 10.1093/cei/uxac086

Figure Lengend Snippet: IgG deposition and the distribution of C3, MAC, and CD16 in the brain tissue of the pups from H-ENO1Ab dams. All the pups were obtained as depicted in . (A) Representative images of IgG deposition in the brain tissues from the pups of H-ENO1Ab dams and those of the control group on P10 and P40 ( n = 4/group) after immunofluorescence (IF) staining with FITC-conjugated goat anti-mouse IgG (green, ×200). (B) Representative images for the colocalization of IgG and ENO1, NeuN, and CD34 in the brain tissues from the pups of H-ENO1Ab dams and those of the control group on P10 ( n = 4/group) after double IF staining with FITC-conjugated goat anti-mouse IgG (green) and rabbit anti-ENO1 and NeuN, rat anti-CD34 (red, ×200). (C and E) Representative images of the colocalization of IgG and C3 and CD16 in the brain tissues from the pups of H-ENO1Ab dams and those of the control group on P10 and P40 ( n = 4/group) after double IF staining with FITC-conjugated goat anti-mouse IgG (green) and rabbit anti-C3 and CD16 (red, ×200). (D) Representative images of MAC expression in the brain tissues from the pups of H-ENO1Ab dams and the those of the control group on P10 and P40 ( n = 4/group) after IF staining with mouse anti-C5b-9 (green, ×200). Nuclei were counterstained with DAPI (blue).

Article Snippet: After 2 h incubation at RT followed by repeated washing, the plates were further incubated with diluted horseradish peroxidase (HRP)-labeled goat anti-mouse total IgG (1:200 diluted for TgAb;1:10 000 for ENO1Ab), IgG1(1:1000 for ENO1Ab), IgG2a (1:1000 for ENO1Ab), IgG2b (1:1000 for ENO1Ab), or IgG3 antibodies (1:1000 for ENO1Ab), which were all from Bethyl (USA).

Techniques: Control, Immunofluorescence, Staining, Expressing

Journal: Toxicology Reports

Article Title: Nevada desert dust with heavy metals suppresses IgM antibody production

doi: 10.1016/j.toxrep.2018.01.006

Figure Lengend Snippet: Autoantibody production against (A) myelin basic protein (IgM and IgG) or (B) glial fibrillary acidic protein (IgM and IgG) in adult female B6C3F1 mice following oropharyngeal aspiration exposure to dust samples from NDRA CBN 6. Data are presented as mean ± standard deviation. Sample size for each group was 5–6 animals. The (*) indicates a response statistically different from the 0 mg/kg group (p < 0.05).

Article Snippet: Monoclonal antibodies coupled to fluorochromes specific for the following markers were used at a concentration of 1 μg/10 6 cells: anti-mouse CD25-FITC, rat IgG1-PE isotype control, rat IgG2b-AF647 isotype control, and rat IgG2b-FITC isotype control (BD Pharmingen, San Diego, CA, USA).

Techniques: Standard Deviation